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Maize single cell rna-seq

WebAbstract. Since the first publication a decade ago describing the use of single-cell RNA sequencing (scRNA-seq) in the context of cancer, over 200 datasets and thousands of scRNA-seq studies have been published in cancer biology. scRNA-seq technologies have been applied across dozens of cancer types and diverse array of study designs to … WebFeb 22, 2024 · scRNA-seq of developing maize ears reveals major cell types and developmental markers • scRNA-seq co-expression networks predict genetic redundancy • • Summary Crop productivity depends on activity of meristems that produce optimized plant architectures, including that of the maize ear.

Top Benefits of Using the Technique of Single Cell RNA-Seq

WebMar 1, 2024 · Single cell RNA‐seq (scRNA‐seq) has been highlighted as a powerful tool for the description of the human cell transcriptome, but the technology has not been broadly applied in plant cells. WebSingle-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery. / Xu, Xiaosa; Crow, Megan; Rice, Brian R et al. In: Developmental cell, Vol. 56, No. 4, 22.02.2024, p. 557-568.e6. Research output: Contribution to journal › Article › peer-review hatch creek state forest ny https://ltemples.com

Single‐cell RNA‐seq describes the transcriptome landscape and ...

WebSep 8, 2024 · Developed is an efficient 3' RNA-seq method, that is, simplified poly(A)-anchored sequencing (SiPAS V2). The present method specifically switches next-generation sequencing adapters in a library, so that an R1 end reads a non-poly(T) end of the library during sequencing, which is more suitable for the standard PE150 … WebApr 28, 2016 · A novel virus, tentatively named Maize Yellow Mosaic Virus (MaYMV), was identified from the field-grown maize plants showing yellow mosaic symptoms on the leaves collected from the Yunnan Province of China by the deep sequencing of small RNAs. The complete 5642 nucleotide (nt)-long genome of the MaYMV shared the highest nucleotide … WebNov 16, 2024 · Single-cell genomics has slowly advanced in plant research. Here, we introduce a generic procedure for plant nuclei isolation and nanowell-based library preparation for short-read sequencing. This plant-nuclei sequencing (PN-seq) method allows for analyzing several thousands of genes in thousands of individual plant cells. In … hatch croke and associates

Recent advances in differential expression analysis for single-cell RNA ...

Category:Single-cell RNA sequencing of developing maize ears facilitates ...

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Maize single cell rna-seq

Single-cell RNA sequencing of developing maize ears facilitates ...

WebDifferential expression (DE) analysis is a necessary step in the analysis of single-cell RNA sequencing (scRNA-seq) and spatially resolved transcriptomics (SRT) data. Unlike traditional bulk RNA-seq, DE analysis for scRNA-seq or SRT data has unique characteristics that may contribute to the difficulty of detecting DE genes. However, the ... WebApr 19, 2024 · Compared with traditional sequencing, single-cell RNA sequencing (scRNA-seq) technology, as the name implies, refers to the sequencing of a single genome for genomic or transcriptomic information that can reveal heterogeneity between cell populations. scRNA-seq methodologies have overcome the challenges of aggregate …

Maize single cell rna-seq

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WebFeb 21, 2024 · As an emerging sequencing technology, single-cell RNA sequencing (scRNA-Seq) has become a powerful tool for describing cell subpopulation classification and cell heterogeneity by achieving high-throughput and multidimensional analysis of individual cells and circumventing the shortcomings of traditional sequencing for … WebCrafting a blueprint for single-cell RNA sequencing Highlights High-throughput single-cell RNA sequencing (scRNA-Seq) offers huge potential to plant research. Optimization of a cell-isolation procedure is critical. Cell numbers and sequencing depth per cell must be balanced to maximize results.

WebDec 1, 2024 · Single-cell RNA-seq of maize root tips and identification of cell types To perform scRNA-seq on maize inbred line B73, sterilized seeds were germinated with nitrate (nitrate+) or without nitrate (nitrate-) for four days. The primary root length was greater under N-deficit than under N-sufficient conditions (Fig. S1). WebSingle-cell RNA sequencing reveals the landscape of maize root tips and assists in identification of cell type-specific nitrate-response genes Author: ... We profiled the transcriptomes of >7000 cells derived from root tips of maize seedlings grown on media with or without nitrate, and identified 11 major cell types or tissues and 85 cell type ...

WebDec 14, 2024 · These single-cell transcriptomic analyses of the shoot apex yield insight into the processes of stem-cell function and cell-fate acquisition in the maize seedling and provide a valuable scaffold on which to better dissect the genetic control of plant shoot morphogenesis at the cellular level. WebJan 4, 2024 · Aside from rice, several studies have applied scRNA-seq toward crop improvement in maize by highlighting the transcriptional differentiation in maize cells at high resolution. For instance,...

WebMay 20, 2024 · Here, we adapted novoSpaRc 34, a methodology for spatial reconstruction of single-cell RNA-seq data, to generate a 3D single-cell transcriptome atlas of a floral meristem by integrating single ...

WebJan 1, 2024 · High-throughput single-cell RNA sequencing (scRNA-Seq) offers huge potential to plant research. ... Curiously, Satterlee et al. [19] describe a markedly improved cell viability of isolated maize meristem cells by supplementing protoplast isolation solutions with l-arginine and increasing the pH from a fairly standard 5.7 to 7.5. For select ... hatch croke and associates lawton okboot flash ciscoWebFeb 4, 2024 · Single-cell RNA sequencing (scRNA-seq) technology has recently been used to examine the cellular identity and heterogeneity of several complex plant tissues in maize ( Zea mays) (Nelms & Walbot, 2024; Satterlee et al ., 2024; Marand et al ., 2024; Xu et al ., 2024 ), Arabidopsis (Denyer et al ., 2024; Zhang et al ., 2024, 2024b; Satterlee et … hatch crosswordWebNov 12, 2024 · Single-cell RNA-sequencing libraries were prepared using the Chromium Single Cell 3′ reagent kit v3 (10x Genomics, PN-1000075; Pleasanton, CA) following the manufacturer’s protocol. Cells were ... boot flareWebJan 15, 2024 · Xu et al. used single cell RNA sequencing (scRNA-seq) to identify spatial regulators of maize ear development. Over 12,500 individual cells and 28,900 genes were analyzed. 12 meta-clusters were predicted, with inflorescence markers from maize or Arabidopsis used to predict the cell or domain identities of these clusters. boot flare pantsWebFeb 20, 2024 · Background: Maize mesophyll (M) cells play important roles in various biological processes such as photosynthesis II and secondary metabolism. Functional differentiation occurs during M-cell development, but the underlying mechanisms for regulating M-cell development are largely unknown. bootflash ciscoWebOct 9, 2024 · Using the maize protoplast system, we have successfully performed over 200 ChIP-seq experiments for 104 TFs that are expressed in the developing section of the maize leaf, based on previous... hatchcroft middlesex university