WebJun 15, 2024 · Poly(2-hydroxyethyl methacrylate) (pHEMA) as a biomaterial with excellent biocompatibility and cytocompatibility elicits a minimal immunological response from host tissue making it desirable for different biomedical applications. This article seeks to provide an in-depth overview of the properties and biomedical applications of pHEMA for bone … To develop a microfluidic chip for tumorsphere culture and analysis, we adapted our previous single-cell hydrodynamic capture scheme29. This scheme is fabricated with a standard PDMS soft lithographic process molded from a two layer SU-8 master (Supplementary Fig. 1). Figure 1a shows the fabricated device … See more To achieve suspension culture in our single-cell platform, we optimized a poly 2-hydroxyethyl methacrylate (polyHEMA) hydrogel coating process to form non … See more After characterizing coating quality, suspension cell culture was performed on open coated substrate (polystyrene dish with polyHEMA coating) to be compared … See more Single-cell derived sphere formation has been suggested as a surrogate assay for stem cell activity13,14,15,41,42, but such assays often suffer from poor single cell … See more There are considerable similarities between healthy stem cells and CSCs. In particular, canonical stem cell pathways such as Wnt and Notch have been shown … See more
Triple‐Decker Sandwich Cultures of Intestinal ... - Current Protocols
WebFirst, 1.2 g of poly-HEMA (Sigma-Aldrich) was dissolved in 40 mL of 95% ethanol by mixing the solution overnight at 37 °C. Then, 50 μL or 3.2 mL of the poly-HEMA stock solution were added to 96-well plates and 10-cm dishes, respectively, under the tissue culture hood; the plates and dishes were swirled for 10 min using a plate rotator. WebUSA) culture. A solution containing 20 mg/ml polyHEMA in 95 % ethanol was made, and 0.95 ml/mm2 of this solution was pipetted into 35 mm wells. The plates were allowed to sit, partially covered, until the ethanol evapo-rated and the polyHEMA had solidified. Periodically, the plates were rocked by hand to help ensure even coating. side dish for aloo paratha
Single cell suspension culture using polyhema coating for anoikis …
WebJun 21, 2024 · To facilitate cell aggregation, each microwell is coated with polyHEMA , which is a biocompatible hydrogel used as a non-adherent coating material for … WebOct 23, 2013 · PolyHEMA creates a synthetic hindrance for the cells and inhibits them from attaching to the plates . Cells thus form 3D structures by attaching to each other. For the PH7d (polyHEMA 7 days) model, the compound libraries were pipetted to the coated plates (10 µl/well), and 40 µl of cell-suspension/well were then added to plates. WebMar 16, 2016 · To investigate the underlying causes of chemoresistance in malignant pleural mesothelioma, we have studied mesothelioma cell lines as 3D spheroids, which acquire increased chemoresistance compared to 2D monolayers. We asked whether the gene expression of 3D spheroids would reveal mechanisms of resistance. To address this, we … side dish for a seafood boil slaw